I-Bio-Pharm

Izinhlobonhlobo zezinhlelo zokuhlola i-ELISA kanye ne-PCR ziyatholakala ukuze ziqondise inqubo yokukhiqiza ebiza kakhulu futhi ebalulekile yezifo zezilwane

Ukuhlangabezana nezidingo zanamuhla zokuxilonga udokotela wezilwane kanye nokusungula izinto ezintsha ukuze kuhlangatshezwane nezinselele zesikhathi esizayo Njengoba uzama ukuqhubekisela phambili ukuqapha impilo yezilwane namuhla, izindlela ezithuthukisiwe zokuhlola izifo zingasiza. Ama-reagents wamakhemikhali athembekile, izinkundla eziguquguqukayo, nezixazululo zokuhlola ezinelayisensi kubalulekile ekuthuthukisweni kwamazinga amasha okuhlola ezilwane zomnotho. Futhi lezi kufanele zihlanganiswe. Kudingeka futhi kusheshe futhi kunembile. Ngoba ukuxilongwa ngesikhathi kwezilwane ezigulayo kungaqinisekisa impilo yawo wonke umhlambi, ngaleyo ndlela kuvikelwe izithakazelo zezomnotho zamabhizinisi okuzalanisa.

Imikhiqizo ye-Cangzhou Weikang isetshenziswa kakhulu ekwelashweni kwezilwane nasekufakweni komuthi wokugomela wezilwane. Njengoba izidakamizwa zezilwane zidinga ukufakwa inzalo eqinile kanye nokwelashwa kokubulala amagciwane, izinto zokupakisha zazo kufanele zibe nezinga eliphezulu lenhlanzeko nezakhiwo zokuphepha. Amabhodlela ethu epulasitiki ngokulungisa ukwakheka kwawo kanye nenqubo, ukuze kuzuzwe amandla aphezulu, umgoqo ophezulu, ukusebenza kwenhlanzeko ephezulu kanye nezinye izidingo ukuze kuhlangatshezwane nezidingo zokupakishwa kwezidakamizwa zezilwane. Ngesikhathi esifanayo, ukugcinwa nokuthuthwa kwemithi yezilwane kanye nemithi yokugoma nakho kuyimikhakha ebalulekile yokusetshenziswa kwamapulasitiki ashintshiwe. Imithi yokugoma idinga ukufakwa efrijini emazingeni okushisa aphansi ukuze igcine ukusebenza kwayo. Amabhodlela ethu epulasitiki ngokungeza izithako ezikhethekile, njengezinto zokushintsha kwesigaba zisetshenziswa endaweni ethile yokushisa okungaguquki umphumela wokushisa, ukuze kuqinisekiswe ukuphepha kwesitoreji somgomo kanye nokuhamba.

Izinzuzo zokupakishwa kwepulasitiki kwezidakamizwa zezilwane

1 I-Acid-alkali ukumelana futhi imelana nomthelela

Amabhodlela epulasitiki enziwe ngezinto ezifana ne-polyethylene ne-polypropylene. Zinokuqina okuqinile, ukumelana ne-asidi-alkali, nezici zokumelana nomthelela ezivikela ngempumelelo imithi yezilwane.

2 Ukufakwa uphawu okuhle nokudluliswa kokukhanya

Isakhiwo esihle sokuvala sebhodlela lepulasitiki siqinisekisa ukumelana nokungenwa komoyampilo, ukungena komswakama, nokungcoliswa kwamagciwane. Ngaleyo ndlela ukwandisa impilo yeshalofu yezidakamizwa zezilwane. Ngasikhathi sinye, indawo enhle yokuvala iphinde isho ukuthi ayikwazi ukuvulwa noma ingcoliswe kalula.

3 Ukuthutha okulula futhi akulula ukuphuka

Amabhodlela epulasitiki angasindi ukuze kube lula ukuhamba nokuphatha; ngaphezu kwalokho azikwazi ukugqoka futhi azijwayele ukuphuka uma kuqhathaniswa nengilazi noma izinto zobumba.

4 Kulula ukwenza ukucubungula.

Njengoba nje esikhundleni isikhunta, angathola izinhlobo ezahlukene iziqukathi, futhi kulula ukwakha ukukhiqizwa mass

Izici eziyinhloko zamabhodlela okufaka epulasitiki ezidakamizwa zezilwane:

1.Amandla: Ngenxa yemininingwane yokupakishwa kwemithi yezilwane kanye nemithi yokugoma yezilwane, umthamo wempushana mkhulu, ngokuvamile u-500ml-5L; Izitsha zomuthi wokugoma zisukela kwezimbalwa kuye kwezingama-500 ml, ezinomthamo osetshenziswa kakhulu ongu-20 kuye ku-250 ml. Ngokwendlela yokuvala inzalo yamabhizinisi omkhiqizo webhayoloji, amabhodlela okupakisha okugoma angavalwa inzalo ngokushisa okuphezulu kwe-autoclave noma i-irradiation angenziwa ngokwezifiso.

2. Ukubekwa uphawu okuhle: Ukuze kuqinisekiswe ukuthi umuthi awumanzi futhi awuwohloki phakathi nesikhathi sokufaneleka, ibhodlela lesidakamizwa sezilwane kufanele libe nokuvaleka okuhle nokungena, ukuvimbela ithonya lokukhanya, ukushisa, umhwamuko wamanzi, umoya-mpilo, njll. , emthini.

3. Hlangana nezidingo ze-"GMP". Udonga lwangaphakathi lwebhodlela luthintana ngqo nomuthi, futhi impahla yebhodlela kufanele ihlangabezane nezidingo zokupakishwa kwezidakamizwa ukuze kuqinisekiswe ukuphepha komuthi.

4. Umkhawulo we-Microbial. Amabhodlela epulasitiki okwelapha aqinile nawuketshezi ayingxenye yesigaba sezinto zokupakisha, angasetshenziselwa ukupakishwa kwezidakamizwa ngaphandle kokuhlanza nokuvala inzalo, ngakho-ke, kunezidingo eziphakeme zomkhawulo we-microbial, imvelo yokukhiqiza kanye nenqubo yokukhiqiza kufanele ihlangabezane nezidingo zemithetho efanele. 5.Umumo. Ukuma, usayizi kanye nesakhiwo samabhodlela ezidakamizwa zezilwane zepulasitiki kufanele kuhlangabezane nezidingo zezinhlobo ezahlukene zemishini yokugcwalisa yamabhizinisi emithi futhi ahlangabezane nezidingo zemishini yokugcwalisa okuzenzakalelayo esheshayo.

Ukusetshenziswa kwamabhodlela okugoma epulasitiki emaphaketheni emithi yezilwane

1.Umuthi wokugoma we-porcine circovirus ongasebenzi: Ukutheleleka nge-porcine circovirus kungabangela izinhlobonhlobo zezimpawu zomtholampilo, okuhlanganisa i-multisystem failure syndrome ezingulubeni ezilunyuliwe, i-porcine dermatitis nephrotic syndrome, ukuphazamiseka kokuzala ezingulubeni, kanye ne-porcine interstitial pneumonia. Umuthi wokugoma ongasebenzi we-porcine circovirus virus usetshenziselwa ukuvimbela ukutheleleka kohlobo lwe-ring 2 olubangelwa izifo ezihlobene, ezifanele amaviki angaphezu kwama-3 ezingulube kanye nezingulube.

2.I-Mycoplasma Galliscepticum (MG) ukutheleleka uhlobo lwesifo sokuphefumula ezinkukhuni. Ukutheleleka kubonakala ngekoloni ku-cilia kanye namasaka omoya we-tracheal mucosa, kuyilapho kungekho mithambo yegazi ekupheleni kwe-cilia ye-tracheal kanye nezikhwama zomoya.

Ngakho-ke, izidakamizwa zinganciphisa kuphela inani le-Mycoplasma pneumoniae colonizing ku-cilia ye-tracheal kanye namasaka omoya futhi inciphise izimpawu zomtholampilo, kodwa ayikwazi ukuyibulala ngokuphelele. Ngakho-ke, ukutheleleka kwe-Mycoplasma gallinarum ngokuvamile kuyisimo sokutheleleka okuqhubekayo impilo yonke, futhi lapho izinkukhu zingaphansi kwengcindezi, njengokukhuthazwa ukubanda kanye nokugoma, zizobonisa izimpawu zomtholampilo.

3.Umuthi wokugomela ongasebenzi wesifo somlomo nezinkomo ezinkomeni: Isifo samatele ezinkomo sidalwa yigciwane lesifo samatele, esibonakala ngamabhamuza nama-plaque esikhumbeni senselo, uzwane kanye isithende, kanye nezilonda ezifanayo ku-mucosa yomlomo kanye ne-nasal disc kwezinye izinkomo. Umuthi wokugoma we-Foot-and-mouth ongasebenzi wohlobo lwe-O usetshenziselwa ukuvimbela isifo samatele nomlomo uhlobo O ezinkomeni futhi unikeza ukuzivikela ezinsukwini eziyi-15 emva komjovo.